Table 3.
Forward primer | Reverse primer | ||||
Flank | Size (kb) | ID primer | Introduced restriction site | ID primer | Introduced restriction site |
Left | 7 | 5 | Acc65I | 6 | NotI |
Left | 5 | 7 | Acc65I | 6 | NotI |
Left | 3 | 8 | Acc65I | 6 | NotI |
Right | 3 | 9 | NotI | 10 | Eco52I, Acc65I |
Right | 5 | 9 | NotI | 11 | Eco52I, Acc65I |
Right | 7 | 9 | NotI | 12 | Eco52I, Acc65I |