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Primer sets used for construction of double homologous crossover cassettes
| Forward primer | Reverse primer | ||||
| Flank | Size (kb) | ID primer | Introduced restriction site | ID primer | Introduced restriction site |
| Left | 7 | 5 | Acc65I | 6 | NotI |
| Left | 5 | 7 | Acc65I | 6 | NotI |
| Left | 3 | 8 | Acc65I | 6 | NotI |
| Right | 3 | 9 | NotI | 10 | Eco52I, Acc65I |
| Right | 5 | 9 | NotI | 11 | Eco52I, Acc65I |
| Right | 7 | 9 | NotI | 12 | Eco52I, Acc65I |
