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. 2009 Mar 26;4(3):e5018. doi: 10.1371/journal.pone.0005018

Figure 2. Silencing of E6 and E7 showed contribution of the oncoproteins to the pro-migratory phenotype of HeLa cells.

Figure 2

(A) Quantitative RT-PCR analysis of E6 and E7 mRNA level and E6 protein expression in knock-down HeLa cells lines. E6 and E7 expression were respectively knocked-down using the pEBVsiRNA in 100, and 102, 103. Inhibition was statically significant P<0.04. (B) Quantitative RT-PCR analysis of CXCR4 mRNA level in clonesKD with CXCR4- specific probe. ***P<0.01 and **P<0.05. (C) Cell invasion was measured using a Matrigel assay in E6KD (100) and E7KD (102, 103) and compared to migration of a control HeLa clone (scrambled sequence). All Experiments were conducted in presence of Hygromycin (125 µg/mL) with DMEM+10% FCS; DMEM+SDF-1α (100 ng/mL); CDF and DMEM+SDF-1α (100 ng/mL). Three independent experiments with three chambers each time were performed. **P<0.05, for a statistically true difference, as compared to the control clone with DMEM+FCS. # P<0.05 and ## P<0.01 compared to the control clone with DMEM+SDF-1α. ° P<0.05 and °° P<0.01 compared to the control clone with CDF and DMEM+SDF-1α.