Figure 5. Cidofovir abrogates TC-1 cell lung metastasis in vivo in a CXCR4 dependent manner.

In the two first groups, TC-1 cells were either untreated or pre-treated for 6 days with 10 µM Cidofovir prior to vein tail injection. In the third group, mice were systemically treated with Cidofovir (100 mg/Kg) 2 h before TC-1 cell injection and then on day at day 2, 4, 6, 8. Lungs were collected and processessed for histology. (A) Representative Hematoxylin-Eosin-Saffranin (HES)-stained sections of lungs of mice are shown. Metastases are delineated by a dashed line. b = bronchi, v = vessel (B) Quantitative assessment of lung macro-metastasis was performed as previously described ***P<0.01 as compared to untreated group. (C) CXCR4 immunostaining was performed as previously described.