Figure 7. p160ROCK inhibition reduces HeLa migration in vitro and TC-1 cell lung metastasis in vivo.

Matrigel assay were performed as previously described with HeLa cells pre-treated for 30 min 10 µM Y-27632. *P<0.05. (B) TC-1 cells were harvested and injected as previously described. Before injection to the mice, TC-1 cells were un-treated or pre-treated for 30 min. with 10 µM Y-27632. Lungs were collected, paraffin-embedded, fixed and cuted into sections. (A) Sections were stained with Hematoxylin-Eosin-Saffranin (HES) and (B) Quantitative assessment of lung macro-metastasis was performed as previously described. ***P<0.01 as compared to untreated group.