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. Author manuscript; available in PMC: 2009 Mar 19.
Published in final edited form as: Am J Physiol Cell Physiol. 2008 Mar 5;294(5):C1288–C1297. doi: 10.1152/ajpcell.00033.2008

Fig. 8.

Fig. 8

Effect of a myosin light chain kinase (MLCK) CaM binding peptide on localization of CaM and the time-dependent appearance of Ca2+ sparks in diaphragm. Immunofluorescence localization of endogenous CaM shows that the MLCK CaM binding peptide (10 μM) results in a loss of endogenous CaM (A and B). C: no detectable fluorescence from fibers incubated in secondary antibody alone is shown. Dotted line outlines the cell for visual purposes. D: no significant alteration in the appearance of Ca2+ sparks was observed in fibers incubated with the MLCK CaM binding peptide (squares) and controls (circles).