Table 2. List of antigens tested for presence in NCSs.
| Compartment | Antigen | in NCS |
|---|---|---|
| NPC | Nup153 | +++* |
| Nup62 | + | |
| Nup358 | − | |
| Nup214 | − | |
| Tpr | +/− | |
| WGA | + | |
| Nuclear envelope | Lamin A/C | +++ |
| Lamin A | + | |
| Lamin B1 | (+) | |
| Lamin B2 | + | |
| Emerin | +++ | |
| LAP2β | (+) | |
| Endoplasmic reticulum | Calnexin | + |
| BiP | + | |
| PDI | + | |
| CLIMP63 | + | |
| Sec61 | − | |
| Nucleolus | Nopp140 | − |
| NAP57/dyskerin | − | |
| Fibrillarin | − | |
| Nucleolin | − | |
| UBF1 | − | |
| Nucleoplasm | Coilin | − |
| Pol II CTD S2-P† | − | |
| Pol II CTD S5-P‡ | − | |
| SC35 | − | |
| Progesterone receptor | − | |
| Estrogen receptor | − | |
| Golgi | p115 | − |
| GM130 | − |
*
+++, highly enriched; +, present; +/−, only in some; (+), barely detectable; −, absent.
†
Antibodies specific for the phosphorylated Ser2 of the C-terminal domain of RNA polymerase II, which is characteristic for the initiating enzyme.
‡
Antibodies specific for the phosphorylated Ser5 of the C-terminal domain of RNA polymerase II, which is characteristic for the elongating enzyme.