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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Bone. 2008 Dec 16;44(4):537–546. doi: 10.1016/j.bone.2008.11.021

Figure 4.

Figure 4

Fat distribution in Ebf1−/− mice. Toluidine blue stained proximal tibiae from 4 week-old Ebf1−/− and age-matched littermate control mice. (A) Adipocyte ghosts fill the secondary center of ossification (above the growth plate) in Ebf1−/− mice as compared to controls (B). (C) Increased adipogenesis in Ebf1−/− bone revealed by extensive osmium staining in the bones of 4-week-old mutant bone. (D) No osmium staining was seen in control bone. Extra-osseous fat serves as a control (arrow). (E) White adipose deposition in the subcutaneous fat pads surrounding the flank region is decreased in 4 week old Ebf1−/− (n=10) compared to controls (n=9). Each point represents the mean ± SE. Statistical significance was determined by ANOVA.

*Significant decrease in Ebf1−/− mice, p ≤0.01. (F) Deposition of fat, indicated by Oil-red-O staining, in liver from Ebf1−/− mice as compared to age-matched littermate control (G).