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. Author manuscript; available in PMC: 2009 Oct 21.
Published in final edited form as: Biochemistry. 2008 Sep 30;47(42):11086–11096. doi: 10.1021/bi801198m

Figure 1.

Figure 1

NAMPT activity as a function of pH for the ATP-coupled NMN synthesis reaction (A). Concentrations fixed at 1 µM [14C]NAM, 200 µM PRPP, 1 mM ATP and 5 mM MgCl2 with 30 nM enzyme. NAMPT ATPase activity as a function of pH (B). Concentrations were fixed at 2 mM ATP and 5 mM MgCl2 with 0.75 µM enzyme.