. Author manuscript; available in PMC: 2009 Oct 21.

Published in final edited form as: Biochemistry. 2008 Sep 30;47(42):11086–11096. doi: 10.1021/bi801198m

Table 1.

Kinetic parameters of human NAMPT for both coupled and non ATP-coupled reactions.

	Non ATP-coupled NMN synthesis		ATP-coupled NMN synthesis

	No Pi	Pi
K_m
NAM (nM)	855 ± 22	235 ± 17	5 ± 2
PRPP (µM)	7.2 ± 0.6	11.7 ± 0.8	0.63 ± 0.03
ATP (Pi)^*(mM)		0.18 ± 0.02^*	7.4 ± 1.5
k_cat (min⁻¹)	0.082 ± 0.001	0.085 ± 0.001	0.46 ± 0.09
k_cat/K_m (M⁻¹ s⁻¹)^†	1.60 ± 0.06 × 10³	6.1 ± 0.5 × 10³	1.8 ± 0.9 × 10⁶

K_m of Pi for the non ATP-coupled NMN synthesis.

^†

k_cat/K_m regarding to NAM.