Table 2.

Inhibition results for both coupled and non ATP-coupled reactions.

	Non ATP-coupled NMN synthesis		ATP-coupled NMN synthesis*
	PRPP	NAM	PRPP	NAM
Ki
FK866 (pM)	730 ± 75 (9863)†	5000 ± 500 (171)	10 ± 1 (63000)	150 ± 18 (33)
5P-DADMe-NMN (µM)	0.53 ± 0.02 (14)	7.1 ± 0.2 (<1)	0.160 ± 0.015 (4)	2.5 ± 0.2 (<1)
NAD+ (µM)		> 100	0.140 ± 0.014 (5)	2.1 ± 0.2 (<1)
NADH (µM)		> 100	0.22 ± 0.02 (3)	3.2 ± 0.2 (<1)

^*K_i determined at 2.5 mM ATP.

^†Ratio K_m/K_i corresponding.