. Author manuscript; available in PMC: 2009 Oct 21.

Published in final edited form as: Biochemistry. 2008 Sep 30;47(42):11086–11096. doi: 10.1021/bi801198m

Table 3.

Comparison of kinetic parameters for human NAMPT and Salmonella typhimurium NAPT.

	Non ATP-coupled NMN/NAMN synthesis		ATP-coupled NMN/NAMN synthesis

	NAMPT	NAPT	NAMPT	NAPT
K_m
NAM (nM)	855 ± 22	290000 ± 50000	5 ± 2	1500 ± 300
PRPP (µM)	7.2 ± 0.6	4500 ± 2200	0.63 ± 0.03	22 ± 3.3
k_cat (min⁻¹)	0.082 ± 0.001	17 ± 3	0.46 ± 0.0.09	174 ± 4
k_cat/K_m (M⁻¹ s⁻¹)^†	1.60 ± 0.06 × 10³	1.0 ± 0.4 × 10³	1.8 ± 0.9 × 10⁶	1.9 ± 0.6 × 10⁶

^†

k_cat/K_m regarding to NAM/NA.