Figure 7.

Coexpression of PDCD5 and Tip60 accelerate DNA damage-induced apoptosis. (A) U2OS cells were transfected with PDCD5 and Tip60 plasmids alone or together. After 24 hours, they were treated with UV irradiation for 12 hours. Then, cells were stained with FITC-Annexin V, and the percentage of apoptosis was determined. The data shown are from a representative experiment. (B) Cell treatment was the same as in (A). Western blots analyze the presence of PARP (full-length or cleaved) and actin. (C) U2OS cells were transfected with the indicated siRNA and plasmids. After 24 hours, they were treated and analyzed as in (A). The data shown are from a representative experiment. (D) As in (C), except that total cell extracts were analyzed by Western blot for the presence of PARP (full-length or cleaved) and actin.