Figure 5.
Basic fibroblast growth factor synergizes with activin A to induce serum-dependent PCC proliferation. PC-3 cells were plated at 40% confluence in medium ± 1% serum for 24 hours before being treated with EGF, IGF-1, and bFGF (10 or 25 ng/ml) ± activin (2.5 ng/ml). Cell proliferation was assessed using the MTS assay after 3 days. Results are presented as a mean percentage of untreated control ± SEM; n = 4(#significantly different from 1% serum control without activin A, P < .01; *significantly different from 1% serum control without activin A, P < .01; @significantly different from 1% serum control with activin A, P < .001).