Figure 6.
Combination of R13 and R28 inhibits lung colonization of GTL-16-luc cells and increases survival in nude (nu/nu) mice. After intravenous injection of (1.25 x 106 cells per mouse) GTL-16-luc cells, bioluminescence images were acquired after luciferin administration (150 mg/kg, intraperitoneally) (A). Tumor recurrence and survival was observed for 170 days after cell injection. Antibodies were administered once weekly from day - 1 to day 21, and animals were continuously imaged every 2 weeks. To reduce stress on the animals, control antibodies 1B711 or R13/28 were injected 24 hours before mice were challenged with GTL-16 cells. Log-rank (Mantel-Cox) comparison of survival plots indicated 80% survival of R13/R28 group versus 0% of 1B711 group at day 170 (P < .001). Please note that at day 70, all mice in R13/28 group still remained tumor-free when compared with 1B711 group (B). Bar, treatment period of 21 days.