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RCK/p54 restricts HIV-1 mRNA association with polysomes. Cytoplasmic extracts from HeLa cells that were transfected with the indicated siRNA and infected with HIV-1-VSVG-luc were run on glycerol gradient (7% to 47%). Fractions were collected and their RNA contents were monitored by measuring absorbance at 254 nm. HIV-1 mRNA (top panel) and Hdm2 mRNA (lower panel) were quantified in all the fractions by Q-RT-PCR using specific oligonucleotides.
