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. 2009 Jan 5;4:2. doi: 10.1186/1749-8104-4-2

Figure 1.

Figure 1

Generation of Nkx6.1::IRES::eGFP mice and embryonic expression of eGFP in the spinal cord. (A) Targeting strategy for the generation of Nkx6.1::IRES::eGFP knock-in mice. (B) Southern blotting to determine wild type (12.5 kb) and targeted (15.5 kb) alleles at the Nkx6.1 locus. (C-F) Expression of Sox3, Nkx6.1, eGFP and Isl1/2 proteins in the e9.5 neural tube of Nkx6.1::IRES::eGFP+/- mice. Nkx6.1 and eGFP proteins are expressed in Sox3+ progenitors (C-E). eGFP is also expressed by MNs (F; ventral Isl1/2+ cells). (G-J) eGFP is expressed in Nkx6.1+ neural progenitors (G, H) and in mature ventral neuronal subtypes such as V2a interneurons (I; Chx10+ cells), and MNs (J; ventral Isl1/2+ cells) at e10.5.