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. 2009 Jan 5;4:2. doi: 10.1186/1749-8104-4-2

Figure 7.

Figure 7

Clones derived from a pMN-restricted progenitor are not committed to the motor neuron fate. (A) Diagram of the experimental strategy used to test the commitment of pMN-restricted clones. (B-G) Staining of pMN-restricted clones for Nkx6.1 (B, E), Olig2 (C, F) and merge panels with Nkx6.1 (red), Olig2 (blue), and eGFP (green) (D, G). Nkx6.1 and Olig2 label motor neuron progenitors in control (B-D) and BMP4-treated (E-G) clones. Both Nkx6.1 and Olig2 are downregulated in BMP4-treated motor neurons clones. eGFP labels MNs. (H-J) Staining of MNs for Isl1/2 and eGFP in control (H) and BMP4-treated clones (I, J). Several Isl1/2+ Tuj1+ neurons are not labeled with eGFP in BMP4-treated cultures (I, J; white arrows). (K-M) Immunhistochemistry for Brn3a (K), Isl1/2 (L) and merge panel (M) of BMP4-treated MN clones. Some Isl1/2+ neurons also express Brn3a (M; yellow cells, white arrow).