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. 2009 Feb 18;2:2. doi: 10.1186/1756-8935-2-2

Figure 5.

Figure 5

Deletion of SGF73 results in a global increase in H2B ubiquitin levels similar to deletion of UBP8, but does not affect H3 acetylation. A. Flag western blot analysis of H2Bub levels: Lane 1 H2BK123R, Lane 2 UBP8Δ, Lane 3 SGF73Δ, Lane 4 SGF73Δ, Lane 5 wildtype. The indicated bands represent FlagH2B, FlagH2Bub and FlagH2BubHA. B. Quantification of H3 lysine 9 Western blot signal demonstrates that deletion of SGF73 does not reduce global histone H3 lysine 9 acetylation when compared with deletion of GCN5.