Figure 5.

Differential effects of ER stress on surface expression of various glycosylated proteins. EL4 cells were incubated in high glucose (4.5 mg/ml) medium (dotted line) or in medium lacking glucose (green) or supplemented with 2.5 mM of palmitate (orange) for 18 hours. (A) Effect of glucose starvation or palmitate treatment on surface expression of glycosylated proteins. Surface expression of H2K^b, H2D^b, CD32, CD45.2, TCR-β and CD5 (Ly1) was determined by flow cytometry analysis. Representative histograms of one of three independent experiments are depicted. (B) Comparative effect of palmitate treatment and glucose starvation on surface expression of glycosylated proteins. Bars represent % of MFI intensity in glucose-starved (green) or palmitate-treated (orange) cells relative to control cells (dotted line). Bars represent the mean and SD from three independent experiments. *P < 0.05 and **P < 0.01 when comparing no glucose or palmitate with control conditions.