Table 3.

Features of neural and mesenchymal differentiation presented by glioblastoma cells

fg	GBM1	GBM2	GBM3	GBM4	GBM8	GFAP+NNP p0	GFAP+NNP p1
Aggregated GBM cells with multilineage phenotype	100	100	100	100	100	no	no
5d Multilineage	100	100	95–100	100	83–87	0–1	0–1
5d NI	0	0	0–2	0	3–6	1–2	1–2
5dMES	0	0	0–2	0	3–5	0	5–8
5dGLIA	0	0	0–3	0	6–10	44–46	45–49
5dNEURO	0	0	0	0	0–3	54–56	47–50
5d not classified	0	0	0–2	0	3–5	0–1	0–1
20d Multilineage	19–23	72–75	12–15	42–46	18–22	0	0
20dNI	21–25	8–12	23–25	11–15	16–20	0	0
20dMES	26–29	3–6	36–40	7–11	4–6	0	5–8
20dGLIA	21–24*	4–9*	15–19*	25–31*	44–51*	48–50*	45–44*
20dNEURO	0	0	0	0	5–7*	50–52*	48–50*
20d not classified	9–12	8–11	8–13	9–12	9–11	0–1	0–1

GBM, glioblastoma;

GFAP+NNP, normal neural progenitors;

NI, neuronal intermediates;

MES, mesenchymal cells;

GLIA, glial cells;

NEURO, neuronal cells;

5d, five days of differentiation (DMEM/F12 N2);

20d, twenty days of differentiation (DMEM/F12 N2);

no, GFAP+NNP did not show stable aggregates.

*After 5 and 20 days of exposure to the differentiation medium glioblastoma cells showed Nestin expression. GFAP+NNP derivatives showed Nestin expression after 5 but not after 20 days of serum starvation.