Table 1.
Oligonucleotides used for amplification of inc genes by PCR.
| Name/Site | Sequence | Target Gene |
|---|---|---|
| DA71 EcoRI | agcaGAATTCttgagatctagaaaagaagc | CT223 C. trachomatis |
| DA97 KpnI | agcaGGTACCaatggtgagtttagcagg | CT223 C. trachomatis |
| DA116 EcoRV | agcaGATATCctacacccgagagccattg | CT223 C. trachomatis |
| DA119 EcoRV | agcaGATATCctaattagccgttttcagatt | CT223/179 C. trachomatis |
| DA121 EcoRV | agcaGATATCctactcttctatctgctcttt | CT223/91 C. trachomatis |
| DA122 EcoRI | agcaGAATTCatggagcttaaagctttagag | CT223/56 C. trachomatis |
| DA76 BamHI | agcaGGATCCttattttttacgacgtgc | CT229 C. trachomatis |
| DA99 KpnI | agcaGGTACCaatgagctgttctaataa | CT229 C. trachomatis |
| DA98 BamHI | agcaGGATCCatgagtactactattgg | CT228 C. trachomatis |
| DA74 PstI | agcaCTGCAGctaagaagcttggttgtc | CT228 C. trachomatis |
| DA 131 EcoRI | agcaGAATTCatgtcttatcttttttcc | CT227 C. trachomatis |
| DA 132 EcoRV | agcaGATATCtcatgagacacttatcac | CT227 C. trachomatis |
| DA 129 EcoRI | agcaGAATTCatgttggccttttttcga | CT226 C. trachomatis |
| DA130 EcoRV | agcaGATATCttatatcagactttccaa | CT226 C. trachomatis |
| DA127 EcoRI | agcaGAATTCatggtggctaacaactttatt | CT225 C. trachomatis |
| DA128 EcoRV | agcaGATATCttaatcccacccatgttt | CT225 C. trachomatis |
| DA125 EcoRI | agcaGAATTCatgagttttgttggaagt | CT224 C. trachomatis |
| DA126 Xhol | agcaCTCGAGctaatcattgggaaatga | CT224 C. trachomatis |
| DA34 EcoRI | agcaGAATTCatgacaacgcctactact | incA C. trachomatis |
| DA21 EcoRV | agcaGATATCctaggagctttttgtggg | incA C. trachomatis |
| DA22 EcoRI | agcaGAATTCggcaacgttatgacgtc | incC C. trachomatis |
| DA23 EcoRV | agcaGATATCttagcttacatatatttg | incC C. trachomatis |
| JL003 EcoRI | agcaGAATTCatgacagtatccacaaa | incA C. caviae |
| JL010 EcoRV | agcaGATATCacttaactatctttatc | incA C. caviae |
| JL007 EcoRI | agcaGAATTCatgtcaacaacaccatc | incB C. caviae |
| JL006 EcoRV | agcaGATATCttaagattctgtttgat | incB C. caviae |
| JL014 EcoRI | agcaGAATTCatgacctctgtaagaga | incC C. caviae |
| JL013 EcoRV | agcaGATATCtaaatgtccggtaggag | incC C. caviae |
| DA114 EcoRI | agcaGAATTCatggtgagcaagggcga | GFP |
| DA115 EcoRV | agcaGATATCctacttgtacagctccatg | GFP |
The restriction sites built into oligonucleotides for cloning purposes are shown in capital letters.