Table 1.
Strains or plasmids | Description* | Reference |
---|---|---|
Strains | ||
Escherichia coli DH5α | General cloning host | [36] |
E. coli ET12567/pUZ8002 | Strain used for E. coli/Streptomyces conjugation | [34] |
E. coli BL21 (DE3) | Strain used for the expression of SgcR3 protein | Novagen |
Bacillus subtilis CMCC(B) 63501 | Strain used for C-1027 bioassays | [35] |
Streptomyces globisporus C-1027 | Wild type C-1027 producing strain | [1] |
S. globisporus R3KO | S. globisporus C-1027 with disruption of sgcR3, Thr | This work |
Plasmids | ||
pUC18 | General cloning vector, Apr | [36] |
pET-16b | E. coli expression vector, Apr | Novagen |
pKC1139 | E. coli/Streptomyces shuttle vector, Amr | [30] |
pOJ260 | Suicide vector nonreplicating in Streptomyces, Amr | [30] |
pSET152 | Streptomyces integrative vector, Amr | [30] |
pL646 | pSET152 derivative plasmid containing ermE*p and the ribosome-binding site of the tuf1 gene upstream atrAc gene, Amr | [37] |
pOJR3KO | pOJ260 derivative plasmid with the disruption of sgcR3, Thr | This work |
pKCR3 | pKC1139 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr | This work |
pSETR3 | pSET152 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr | This work |
pLR3 | pL646 derivative plasmid containing 1,188 bp coding region of sgcR3 instead of atrAc, Amr | This work |
pKCR1R2 | pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 including its native promoter, Amr | This work |
pKCER1R2 | pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 under the ermE*p, Amr | This work |
*Abbreviations: Apr, ampicillin resistance; Amr, apramycin resistance; Thr, thiostrepton resistance.