Table 1.

Bacterial strains and plasmids used in this study

Strains or plasmids	Description*	Reference
Strains
Escherichia coli DH5α	General cloning host	[36]
E. coli ET12567/pUZ8002	Strain used for E. coli/Streptomyces conjugation	[34]
E. coli BL21 (DE3)	Strain used for the expression of SgcR3 protein	Novagen
Bacillus subtilis CMCC(B) 63501	Strain used for C-1027 bioassays	[35]
Streptomyces globisporus C-1027	Wild type C-1027 producing strain	[1]
S. globisporus R3KO	S. globisporus C-1027 with disruption of sgcR3, Thr	This work
Plasmids
pUC18	General cloning vector, Apr	[36]
pET-16b	E. coli expression vector, Apr	Novagen
pKC1139	E. coli/Streptomyces shuttle vector, Amr	[30]
pOJ260	Suicide vector nonreplicating in Streptomyces, Amr	[30]
pSET152	Streptomyces integrative vector, Amr	[30]
pL646	pSET152 derivative plasmid containing ermE*p and the ribosome-binding site of the tuf1 gene upstream atrAc gene, Amr	[37]
pOJR3KO	pOJ260 derivative plasmid with the disruption of sgcR3, Thr	This work
pKCR3	pKC1139 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr	This work
pSETR3	pSET152 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr	This work
pLR3	pL646 derivative plasmid containing 1,188 bp coding region of sgcR3 instead of atrAc, Amr	This work
pKCR1R2	pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 including its native promoter, Amr	This work
pKCER1R2	pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 under the ermE*p, Amr	This work

*Abbreviations: Ap^r, ampicillin resistance; Am^r, apramycin resistance; Th^r, thiostrepton resistance.