Notch pathway is up-regualted in GR cells.
A: Real-time RT-PCR was used to quantify Notch pathway genes at the mRNA levels in GS and GR cells. *, P<0.05, **, P<0.01 compared with GS.
B: Western blot analysis showing the expression of Notch pathway related proteins.
C: Immunofluorescence staining for the expression and cellular localization for Notch-2, Notch-4 and Jagged-1.