Down-regulation of Notch-2 and Jagged-1 inhibited cell migration and invasion, and reduce attachment and detachment of GR cells. Abbreviations: GS, Gemcitabine-sensitive; GR, gemcitabine-resistant. CS, control siRNA; NS, Notch-2 siRNA; JS, Jagged-1 siRNA. *, P<0.05, **, P<0.01 compared with control.
A: GR cells showed increased cell attachment and detached.
B: Down-regulation of Notch-2 or Jagged-1 in GR cells dramatically decreased the attachment and detachment of cells.
C: Transfection of GR cells with Notch-2 siRNA or Jagged-1 siRNA inhibited the migration and invasion.