Figure 3.

Rnd3 expression does not alter cell growth of mutant B-RAF expressing invasive melanoma cells. A, Quantitation of cell entry into S-phase. Inducible knockdown cells were labeled for 7 hours with EdU. DNA synthesis was analyzed by flow cytometry. Depicted are representative traces from one of three independent experiments. B, Melanoma cells cultured in complete medium 72 hours ± 0.1 μg/ml dox to induce expression of Ctl, Rnd3^#1 or Rnd3^#2 shRNA. DNA was stained with propidium iodide and analyzed by flow cytometry. The graph represents the percentage of cells in G₀/G₁, S, and G₂/M phases of the cell cycle. Shown are means ± SD from three independent experiments. C, Cell growth of inducible knockdown cells cultured in complete growth medium ± dox. Equal numbers of cells plated at day 0 ± dox were harvested and counted at days 3 and 7. Shown are means ± SD from one experiment performed in triplicate. A total of three independent experiments were performed.