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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Cancer Res. 2009 Feb 24;69(6):2224–2233. doi: 10.1158/0008-5472.CAN-08-3201

Figure 5.

Figure 5

Melanoma invasive outgrowth is inhibited following Rnd3 knockdown. Spheroids formed from Ctl, Rnd3#1 and Rnd3#2 shRNA WM793TR cells embedded in a 3-D collagen gel for 4 days. A, Phase contrast pictures displaying spheroid morphology at day 0 and day 4 in ± dox. B, Quantitation of spheroid outgrowth shown in (A). Spheroid surface area measured at day 4 normalized to its area at day 0. C, Cell viability within spheroids after 4 days embedded in a collagen gel, live cells stain green (calcein-AM) and dead cells stain red (ethidium bromide).