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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: Biochemistry. 2009 Feb 10;48(5):1036–1046. doi: 10.1021/bi801792q

Figure 5. Effect of Heparin trap on the ATP hydrolysis of UvsW in the presence of circular single-stranded M13 DNA and 60mer oligonucleotide.

Figure 5

ATP hydrolysis of UvsW measured by the coupled spectrophotometric method with different order of additions. A. In control reactions, UvsW (47 nM) and ATP (1 mM) were preincubated together followed by the simultaneous addition of ssM13 DNA (25 µM bases) and heparin at the indicated concentrations. Otherwise, UvsW was preincubated with ssM13 DNA (25 µM bases) followed by the simultaneous addition of ATP (1 mM) and heparin at the indicated concentrations. B. Same order of addition as A. except that d(N)60 was used in place of ssM13 and only a single concentration of heparin was used.