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KM and kcat for Various ssDNA Substrates
| Substrate | KM(µM) | kcat(sec−1) |
|---|---|---|
| d(N)30 | 5.8 ± 0.5 | 19.7 ± 0.3 |
| d(T)30 | 3.8 ± 0.2b | 0.47 ± 0.06b |
| d(A)30 | N.D.a | 0.02 ± 0.01bc |
| d(C)30 | N.D.a | 0.05 ± 0.03bc |
| d(AC)15 | 8.7 ± 1.2 | 5.4 ± 0.2 |
| d(TC)15 | 5.6 ± 1.5 | 3.2 ± 0.2 |
| ssM13 | 1.9 ± 0.3 | 75 ± 3 |
| ----d | N.D.a | 0.00 ± 0.02 |
Not determined
Value obtained using TLC plate assay.
The kcat reported is an apparent kcat at saturating ATP and ssDNA concentrations
no addition of ssDNA
