Table 1.

Summary of some recent studies using mass spectrometry-based targeted protein quantification

Specimen	Quantitative study	Sample preparation	MS platform	Reference
plasma	Major human plasma proteins	Immunodepletion	LC ESI Triple Q / MRM	64
	Low abundance human plasma proteins	Immunodepletion+SCX	LC ESI Triple Q / MRM	65
	Selected N-glycoproteins in human plasma	N-Glycopeptide enrichment	LC ESI Triple Q / MRM	53
	Liver-derived proteins in human plasma	Immunodepletion	LC ESI Triple Q / MRM	66
	Biomarker candidates in mouse model of breast cancer	Immunodepletion+SISCAPA	LC ESI Triple Q / MRM	95
Serum	Selected N-glycoproteins in human serum	N-Glycopeptide enrichment	LC MALDI TOF/TOF	51
	Protein markers of lung cancer in human serum	Immunoprecipitation	LC ESI Triple Q / MRM	111
	Growth hormone biomarkers in human serum		LC ESI Triple Q / MRM	68
	C-reactive protein for rheumatoid arthritis in human serum	Immunodepletion+size exclusion chromatography (SEC)	LC ESI Triple Q / MRM	69
	Intermediate abundance proteins in human serum	Immunodepletion	LC ESI single Q	94
	Prostate-Specific Antigen in human serum	SCX	LC ESI Triple Q / SRM	109
CSF	Biomarker candidates of Alzheimer's disease and Parkinson's disease	SCX	LC MALDI TOF/TOF	72
Urine	Staphylo coccus aureus superantigenic toxins	1D gel	LC ESI Q/TOF	62
Tissue	Biomarker candidates in mouse model of breast cancer		LC ESI Triple Q / MRM	95
	Human liver alcohol dehydrogenase ADH1C1 isoenzyme		LC ESI Triple Q / MRM	63
	Selected proteins of Postsynaptic Density from rat brain	1D gel	LC ESI ion trap / SRM	73
Cell lysate	Yeast proteins involved in gene silencing, cell cycle-dependent phosphorylation of Ser-1126 of human separase protein	1D gel	LC ESI ion trap / SRM	46
	Cyclin-dependent kinases	Immunoprecipitation	LC ESI ion trap / SRM	48
	virulence factors of the gram-positive bacterium Streptococcus pyogenes	Isoelectric focusing	LC ESI Triple Q / MRM	60
	Biomarker of Candida albicans	SCX	LCMALDI Triple Q / MRM	77