Table 1.
Kinase | Inhibitor Designation | Inhibition Constant | Concentration Used | Reference |
---|---|---|---|---|
AMPK | Compound C | Ki=109nM | 1μM | [14] |
STAT3 | S3I-201 | Inhibits STAT3-dependent tumor growth at 100μM | 100μM | [15] |
PI3K | 522DB13D | IC50=4.5μM | 20μM | [16] |
Akt | 1L6HCI | IC50=5μM | 10μM | [17] |
p38 | 24C44F5P | IC50=35nM | 100nM | [18] |
Kinase inhibitors were used within the present cell culture model at or in excess of previously identified inhibitory concentrations. In addition, the PPARγ antagonist, G3335 (KD=8μM), was applied at 30μM [19]. Concentration-dependent inhibition of the target kinases were confirmed by immunoblot (data not shown).