Table 5.

Probability that g20 sequence clusters are non-random with respect to the temperature at the site from which they were collected.

Environment	Temperature (°C)	# Sequences	Unifrac P-value
Sewage	N/A	6	≤ 0.0001
Cold	< 4.99	20	≤ 0.0001
Cool	5–14.99	57	0.2209
Medium	15–21.99	141	0.2296
Warm	22–29.99	467	0.0003
Hot	> 30	3	0.0394

The Unifrac distance metric (Lozupone et al., 2006) was used for the analysis. Temperature values, when not available from the published work, were obtained from the communicating author of the paper in which the g20 sequence was first reported. All but the sequences from moderate temperatures clustered non-randomly (P< 0.05) with respect to the habitat type as defined by temperature.