Fig. 2.
(A) Schematic representation (not to the scale) of Lwt (2233 amino acids) and deletion mutants. N-terrminally FLAG epitope tagged N-terminal 328 amino acids (L N328) were expressed together with Lwt or L C863 or L N889. (B) Analysis of L-L interaction by co-immunoprecipitation assay. 35S-labeled proteins were immunoprecipitated with anti-FLAG antibody and analyzed by SDS-PAGE followed by autoradiography as described in text. The migration positions of full length L and L deletion mutant proteins are indicated. L N328 interacted with Lwt, L C863 and L N889.
