FIGURE 5.

Phenotypes of the mutants lacking mitochondrial NADP⁺-dependent dehydrogenase genes. A, growth of the single mutant carrying pRS415 on SD solid media containing Arg and 0.5, 1.0, or 2.0 mm H₂O₂. B–D, growth of the single (B) or double (C and D) mutants carrying POS5 (pMK2127), 62MTS+yfjB (pMK1723), pRS415, or 62MTS+UTR1 (pMK1722) on SD solid media containing both Arg and 2.0 mm H₂O₂ (B and C), and with neither Arg nor H₂O₂ (D). Mutants carrying pRS415 grew as well as the WT carrying pRS415 on SD solid media containing Arg (data not shown). In the single mutants, each gene is replaced by kanMX4. In the double mutants, POS5 is replaced by HIS3MX6 and each dehydrogenase gene by kanMX4.