. 2009 Mar 20;284(12):7553–7560. doi: 10.1074/jbc.M804100200

TABLE 2.

Kinetic values for the NAD⁺ and NADH of Pos5p, Utr1p, Yef1p, and NADK3

Values were determined by varying the concentrations of NAD⁺ or NADH in the presence of ATP at 5.0 mm (Pos5p, Utr1p, and Yef1p) and 4.0 mm (NADK3), except for the V_max of Utr1p and Yef1p, which was the specific activity assayed in the presence of 1.0 mm ATP and 1.0 mm NAD⁺ or 1.0 mm NADH (7).

	*K_m*		V_max		V_max/K_m
	NAD⁺	NADH	NAD⁺	NADH	NAD⁺	NADH
	mm			μmol.min^-1.mg^-1
Pos5p	5.3	0.49	5.1	59.8	0.98	121
Utr1p	0.5^a	3.9^a	51.5^b	1.2^b	103	0.31
Yef1p	1.9^a	2.0^a	3.3^b	1.2^b	1.7	0.60
NADK3^c	2.4	0.042	23.2	41.2	9.7	981

Data are from Ref. 5.

Data are from Ref. 7.

Data are from Ref. 27.