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. 2009 Mar 20;284(12):7578–7587. doi: 10.1074/jbc.M710013200

FIGURE 2.

FIGURE 2.

LPS-induced IL-12p40 production is regulated by the JNK and the CaM/CaMK-II activated pathways in pSRα-infected monocytes and THP-1 cells. Monocytes (left panel) and THP-1 cells (right panel) were infected twice with control pSRα retroviruses for 24 h each. Cells were treated with (A) JNK inhibitor SP600125 or (B) various inhibitors specific for calcium signaling (EGTA, SKF96365, W-7, and KN-93) at indicated concentrations for 2 h followed by simulation with LPS (1 μg/ml) for another 24 h. The supernatants were analyzed for IL-12p40 production by ELISA. The results shown are mean ± S.D. of four independent experiments.