FIGURE 4.
Nef inhibits SEK1 phosphorylation in LPS-activated monocytes and THP-1 cells. 2 × 106 THP-1 cells (left panel) and monocytes (right panel) were infected twice with either pSRα or pSRα-Nef retroviruses followed by LPS stimulation (1 μg/ml) for 0–60 min. Total proteins were subjected to Western blot analysis for SEK1 phosphorylation using anti-phospho-SEK1 antibodies. To ensure equal protein loading, the blots were reprobed with anti-SEK1 antibodies. Quantitation and normalization as mean densitometry units to the kinase content are shown in the bottom panels. The result shown is a representative of three independent experiments.