FIGURE 2.

A luciferase reporter plasmid, rFS(0.3ex45)-luc, that incorporates the Smad-binding element of intron 1 but not the region upstream of –312 of the rat Fst is activated in response to activin A (1 nm) in αT3-1 but not HEK293T cells. The transfection conditions were the same as those described for Fig. 1. FoxH1 plasmid was included along with Smad2/4. The reported data (arbitrary light units) reflect luciferase activity normalized to β-galactosidase and then calculated relative (Rel) to pGL2 activity obtained from cells treated under the same conditions. The experiments were performed in triplicate, and the reported mean ± S.E. values are from a representative experiment with each cell line (**, p < 0.001 relative to the corresponding untreated basal; #, p < 0.05 relative to basal of the group).