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. 2009 Mar 20;284(12):7631–7645. doi: 10.1074/jbc.M806676200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — FoxL2 associates with the MH2 domain of Smad3. a, HEK293T cells were transfected with vectors for co-expression of FLAG-mFoxL2 with myc-Smad2, myc-Smad3, or myc-Smad4. After 3 days of protein expression, the cells were treated with vehicle or 1 nm activin A for 30 min. Cell lysates were immunoprecipitated (IP) by incubation with agarose-coupled mAb anti-FLAG, and immunoprecipitates were analyzed by Western blots using mAb anti-Myc. Inputs of Myc-tagged Smads (middle panel) and FLAG-mFoxL2 (bottom panel) were validated by Western blot, as shown. IB, immunoblot. b, HEK293T cells were transfected with FLAG-mFoxL2, myc-Smad3-MH1, or myc-Smad3-MH2. Cell lysates containing mFoxL2 were combined with equivalent amounts of those expressing myc-Smad3-MH1 or myc-Smad3-MH2 and then immunoprecipitated by incubation with agarose-coupled mAb anti-FLAG and subjected to Western blot analysis using mAb anti-Myc. Inputs of myc-Smad3-MH1 or myc-Smad3-MH2 (middle panel) and FLAG-mFoxL2 (bottom panel) were validated by Western blot, as shown.