Skip to main content
. 2009 Mar 20;284(12):7733–7745. doi: 10.1074/jbc.M806192200

FIGURE 5.

FIGURE 5.

Enhancement of the human Mus81·Eme1 nuclease function by human Rad54. A, the 3′ DNA flap substrate (6 nm) was incubated with human Mus81·Eme1 (0.13 nm) and without or with human Rad54 (hRad54) (0.5, 1, 2, 4, 8, or 16 nm) at 37 °C for 30 min and then analyzed. B, quantification of the data shown in panel A. C, human Rad54 enhances the nuclease activity of Mus81·Mms4. The 3′ DNA flap (6 nm) was incubated with Mus81·Mms4 (0.25 nm) and the indicated amounts of human Rad54 (4, 8, or 16 nm) or yeast Rad54 (yRad54, 2 or 4 nm) at 37 °C for 30 min and then analyzed. D, quantification of the data shown in C.