Skip to main content
. 2009 Mar 20;284(12):7783–7792. doi: 10.1074/jbc.M806150200

FIGURE 1.

FIGURE 1.

Knockdown of LANP by RNA interference. A, PC12 cells transfected with a pool of siRNA targeting LANP show reduced expression at the protein level as demonstrated by Western blot (WB). As expected, no reduction of LANP is found with control siRNA or cells transfected in a mock manner (no siRNA). Western blot performed with an anti-actin antibody serves as a loading control. B, PC12 cells transfected with siRNA targeting LANP show reduced expression at the RNA level as demonstrated by RT-PCR. Once again, no reduction of LANP is found with control siRNA or cells transfected in a mock manner (no siRNA). In these experiments, the GAPDH message was measured as an internal control. C, quantitative RT-PCR comparing PC12 cells transfected with LANP siRNA and control (C) siRNA. Ct (threshold cycle) value of each sample is normalized to GAPDH and -fold change is relative to control transfected PC12 cells. *, p < 0.0001.