FIGURE 5.

LANP resides at the promoter of the Nf-L gene demonstrated by chromatin immunoprecipitation. A, chromatin bound to LANP was immunoprecipitated from PC12 cell lysates (input) with an anti-LANP antibody (or IgG serving as a control). PCR was performed to amplify the promoter regions of NF-L or two control genes, GAPDH and actin. B, the neurofilament promoter bound to LANP by ChIP was quantified by qPCR by normalizing to one of the controls (GAPDH) with the results graphed as -fold enrichment compared with pulldown by IgG. ^*, p < 0.005.