FIGURE 6.

Knockdown of LANP results in hyperacetylation at the NF-L promoter. A, PC12 cells were transfected with siRNA targeting LANP or control siRNA. 48 h post-transfection, cells were processed for chromatin immunoprecipitation with anti-acetyl H3 (H3 ac), anti-H3 total antibody, or IgG (serving as a negative control). PCR was performed to amplify the promoter region of NF-L and GAPDH (control). Pre-immunoprecipitated lysate served as input. B, qPCR analysis quantified the pulldown. ^*, p < 0.01. This figure is representative of three individually performed transfection experiments.