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. 2009 Mar 20;284(12):7783–7792. doi: 10.1074/jbc.M806150200

FIGURE 8.

FIGURE 8.

Deletion of LANP in mice results in hyperacetylation at the Nf-L promoter and increase in NF-L message. A and B, neurons from LANP mice demonstrate increased acetylation at the Nf-L promoter. Postnatal day 2 mice were sacrificed and brains were processed for chromatin immunoprecipitation with anti-acetyl H3 (H3 ac), anti-H3 total, or IgG (serving as control) antibody. PCR was performed to amplify the promoter region of Nf-L and GAPDH (control). Pre-immunoprecipitated lysate served as input. A, semi-quantitative PCR. B, qPCR analysis quantified the pulldown. *, p < 0.005. This figure is representative of two individually performed chromatin immunoprecipitation experiments. C, neurons from LANP null mice demonstrate an increase in the NF-L message. RNA was isolated from brains of postnatal day 2 wild type (+/+) and LANP-null (-/-) mice. DNase-treated RNA was subjected to quantitative RT-PCR analysis employing primers to amplify NF-L and GAPDH transcripts. Ct value of each sample is normalized to GAPDH and -fold change is relative to RNA from wild-type brain (*, p < 0.01).