TABLE 1.
Fitted binding parameters for two-state conformational exchange reaction See Reaction 1, which shows the two-state reaction with conformational exchange.
| ka1 | kd1 | ka2 | kd2 | KD | X2 | |
|---|---|---|---|---|---|---|
| m–1 s–1 | s–1 | s–1 | s–1 | nm | ||
| IN 220–270 | 5.45 (± 0.10) × 105 | 1.19 (± 0.02) × 10–1 | 4.11 (± 0.02) × 10–3 | 2.75 (± 0.01) × 10–3 | 87.3 ± 2.2 | 8.82 |
| IN | 4.82 (± 0.04) × 104 | 1.45 (± 0.02) × 10–2 | 6.23 (± 0.10) × 10–3 | 1.59 (± 0.03) × 10–3 | 61.2 ± 1.6 | 3.55 |
| IN 220–270/K258A | 1.55 (± 0.01) × 104 | 3.25 (± 0.03) × 10–2 | 4.64 (± 0.03) × 10–3 | 2.69 (± 0.02) × 10–3 | 769.5 ± 9.9 | 0.97 |