FIGURE 2.

Construction of glrA mutant of A. nidulans. A, strategy for homologous recombination into glrA locus to construct glrA deletion mutants. kb, kilobases. B, Southern blot analysis of A. nidulans WT (FGSC A26) (lane 1) and DGR1 (lane 2). Total DNA from strains was digested with PstI before blotting and hybridization. C, strains were grown on MMDN agar plates with or without 10 mm GSH at indicated temperatures for 72 h. Radii of colonies are indicated in mm with S.E. D, conidia of WT (circles) and DGR1 (squares) strains of A. nidulans were spread onto MMDN agar plates with (open) or without (closed) 10 mm GSH and incubated at 30 °C for 120 h. Relative germination rates were determined by setting number of colonies on MMDN agar plates (without oxidant) as 100%. Data are the means of three experiments. Error bars represent S.E. E, mycelia of WT and DGR1 strains of A. nidulans were incubated for 6 h at 30 °C, and intracellular glutathione was determined. Closed and open bars represent reduced and oxidized glutathione, respectively. S.E. are <20%.