TABLE 2.
Putative proteins involved in oxidative responses in A. nidulans identified in two-dimensional PAGE
| No.a | Annotation name | Gene IDb | Genec | -Foldd | tpIe | tMWf |
|---|---|---|---|---|---|---|
| Oxidative stress response | ||||||
| 1 | Thioredoxin reductase | AN3581.3 | trrA | 6.2 | 5.2 | 36.1 |
| 2 | Cytochrome c peroxidase | AN1630.3 | CCP1 | 2.0 | 8.9 | 39.9 |
| 3 | Catalase B | AN9339.3 | catB | 4.8 | 4.9 | 79.2 |
| 4 | Catalase-peroxidase (catalase D) | AN7388.3 | cpeA | 1.2 | 5.9 | 82.1 |
| 5 | Catalase A | AN8553.3 | catA | 1.3 | 7.7 | 60.2 |
| 6 | Manganese superoxide dismutase | AN0785.3 | sodM | 1.1 | 6.2 | 23.4 |
| 7 | Cu,Zn superoxide dismutase | AN0241.3 | SOD1 | 0.9 | 5.8 | 15.9 |
| 8 | Peroxiredoxin/Prx5-like /allergen Asp f3 | AN8692.3 | AHP1 | 4.2 | 5.6 | 18.5 |
| 9 | Peroxiredoxin | AN4301.3 | DOT5 | - | 9.5 | 21.8 |
| 10 | Peroxiredoxin | AN10223.3 | PRX1 | 1.8 | 5.5 | 23.3 |
| 11 | Elongation factor 1B | AN9304.3 | elfA | 2.5 | 5.4 | 24.0 |
| 12 | Hypothetical protein (glutathione S-transferase) | AN6024.3 | gstB | 2.6 | 5.9 | 24.9 |
| 13 | Glutathione reductase | AN0932.3 | glrA | g | 6.2 | 51.6 |
| Other | ||||||
| Up-regulated proteins | ||||||
| 14 | Sulfite reductase | AN1752.3 | - | 1.9 | 5.0 | 114.8 |
| 15 | Glucose-methanol-Choline oxidoreductase | AN7267.3 | - | 3.7 | 5.7 | 59.2 |
| 16 | M20A metallohydrolase family | AN3459.3 | DUG1 | 2.0 | 5.4 | 52.6 |
| 17 | Tropomyosin | AN5686.3 | TPM2 | 2.1 | 5.0 | 18.8 |
| 18 | Proteasome subunit α 2 | AN6726.3 | PRE8 | 2.2 | 5.6 | 30.1 |
| 19 | Zinc binding dehydrogenase | AN7914.3 | - | 2.3 | 5.0 | 36.0 |
| 20 | Reduced viability upon starvation protein 161 | AN8831.3 | RVS161 | 2.8 | 5.6 | 31.2 |
| 21 | Serine hydroxymethyl transferase | AN3058.3 | SHM2 | 1.7 | 7.6 | 50.3 |
| 22 | Ubiquinol cytochrome c reductase core protein 2 | AN8273.3 | QCR2 | 2.3 | 9.0 | 47.6 |
| 23 | Woronin body major protein | AN4695.3 | hexA | 1.9 | 6.7 | 25.1 |
| 24 | Aconitase | AN5525.3 | acoA | 1.7 | 6.0 | 83.1 |
| Down-regulated proteins | ||||||
| 25 | Hypothetical protein | AN3674.3 | - | 0.58 | 4.9 | 58.2 |
| 26 | O-Methyl transferase | AN9233.3 | - | 0.50 | 6.1 | 42.9 |
| 27 | Short chain dehydrogenase | AN0179.3 | TMA29 | 0.35 | 6.2 | 29.3 |
| 28 | UDP-N-acetylglucosamine pyrophosphorylase | AN9094.3 | QRI1 | 0.48 | 6.0 | 55.8 |
| 29 | Pyruvate dehydrogenase complex E2 component | AN6708.3 | LAT1 | 0.46 | 6.1 | 51.8 |
| 30 | S-Adenosylmethionine synthetase | AN1222.3 | SAM2 | 0.61 | 5.3 | 42.2 |
| 31 | Bifunctional purine biosynthesis protein | AN4464.3 | purH | 0.55 | 6.3 | 65.6 |
| 32 | 6-phosphogluconate dehydrogenase | AN3954.3 | GND2 | 0.52 | 5.7 | 54.0 |
| 33 | Phosphoglycerate kinase | AN1246.3 | pgkA | 0.45 | 6.4 | 44.9 |
| 34 | Flavohemoglobin | AN7169.3 | YHB1 | 0.60 | 6.1 | 44.9 |
| 35 | Alcohol dehydrogenase | AN8979.3 | alcA | 0.49 | 7.6 | 37.1 |
| 36 | Peptidyl-tRNA hydrolase | AN10249.3 | PTH2 | 0.60 | 5.5 | 57.5 |
| 37 | ATP synthase α -chain | AN1523.3 | ATP1 | 0.39 | 9.1 | 61.9 |
| 38 | Porin | AN4402.3 | POR1 | 0.41 | 9.0 | 29.9 |
| 39 | Constitutive protein, actin | AN6542.3 | actA | 1.1 | 5.5 | 41.7 |
a
Numbers in this correspond to the protein spots in supplemental Fig. 4 and 5.
b
Gene IDs are according to A. nidulans genome database.
c
Gene names for orthologs in S. cerevisiae are shown when relevant Aspergillus genes were uncharacterized. -, no orthologous gene was identified in S. cerevisiae.
d
Levels of expression in A. nidulans DGR1 (glrA disruptant) were compared with FGSC A26 (wild type). Standard deviations of data were <31%. p < 0.05.
e
Theoretical pI.
f
Theoretical molecular mass.
g
No GlrA was detected in strain DGR1.