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. 2009 Mar 20;284(12):8064–8072. doi: 10.1074/jbc.M808942200

FIGURE 6.

FIGURE 6.

Analysis of the pVP2 cleavage site Ala-441-Phe-442. A, Western blot analysis of proteins expressed in cells infected with rVV VT7LacOI/POLY, VT7LacOI/A441G, VT7LacOI/F442G, and VT7LacOI/A441G/F442G. Infected cultures were harvested at 24, 48, and 72 h pi (hpi). B, QM7 cells were infected with different recombinants VT7LacOI/A441G, VT7LacOI/F442G, or VT7LacOI/A441G/F442G were pulse-labeled with [35S]Met for 1 h at 19 h pi. The radioactivity was chased with by adding with fresh medium containing an excess of cold methionine. Cultures were harvested at 0, 24, 48, and 72 h post-labeling, and the corresponding extracts immunoprecipitated using anti-VP2 serum. The resulting samples were subjected to SDS-PAGE followed by autoradiography. Molecular weight markers (expressed in kDa, left), and bands corresponding to proteins pVP2 and VP2 (right) are indicated.