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. 2009 Mar 20;284(12):8127–8135. doi: 10.1074/jbc.M808815200

FIGURE 1.

FIGURE 1.

SUG-1 interacts with SRC-3 and contributes to RA-induced degradation of SRC-3. A, in MCF7 and HeLa cells, MG132 reverses the RA-induced degradation of SRC-3 and RARα (16 h). B, COS-1 cells were transfected with the FLAG-SRC-3 vector in the absence or presence of SUG-1 and treated or not with RA (1 h). Nuclear extracts were immunoprecipitated with FLAG antibodies and analyzed by immunoblotting. The two upper panels correspond to aliquots (10%) of unprecipitated extracts. C, in MCF7 cells, SUG-1 interacts with SRC-3 in coimmunoprecipitation experiments performed with SRC-3 antibodies. D, immobilized GST and GST-SUG-1 proteins were incubated with extracts from COS-1 cells overexpressing SRC-3. Bound SRC-3 was analyzed by immunoblotting. Lane 1 corresponds to 5% of the loaded material. E, MCF7 and HeLa cells were transfected with control or SUG-1 SMARTpool siRNA (50 nm) and RA-treated for 16 h. Knockdown of SUG-1 was analyzed by immunoblotting as well as the expression and degradation of SRC-3 and RARα. *, a nonspecific band recognized by the RARα antibodies.