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. 2009 Mar 20;284(12):8127–8135. doi: 10.1074/jbc.M808815200

FIGURE 4.

FIGURE 4.

In MCF7 cells, overexpression of SUG-1 blocks the transcription of RARα target genes and the degradation of RARα. A, MCF7 cells were transfected with a vector encoding SUG-1 and were RA-treated. SRC-3 and RARα degradation as well as the efficiency of SUG-1 overexpression were analyzed by immunoblotting. B, overexpression of SUG-1 blocks the RA-induced expression of a DR5-tk luciferase reporter gene. The values are the mean ± S.D. of triplicate experiments. The efficiency of SUG-1 overexpression is shown in the right panel. C and D, overexpression of SUG-1 reduces the RA-induced expression of Cyp26A1, as assessed by qRT-PCR. The results are the average of three experiments that agreed within 15%. E and F, in MCF7 cell knockdown for SUG-1, re-expression of SUG-1 in excess amounts blocks the degradation of RARα and the expression of a DR5-tk-Luc reporter gene. The values are the mean ± S.D. of three independent experiments.