FIGURE 9.

Proposed mechanism by which Ccdc80 bidirectionally regulates adipogenesis. 3T3-L1 preadipocytes express high levels of Ccdc80 upon reaching postconfluence, which are required for the efficient repression of TCF-mediated transcriptional activity after the addition of adipogenic inducers. Ccdc80 also plays a negative role in adipocyte differentiation by a mechanism distinct from inhibition of canonical Wnt signaling. Balanced levels of Ccdc80 are required for the induction of C/EBPα and PPARγ and the subsequent terminal differentiation into mature adipocytes.